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Previously, we have described a linearization procedure for the determination of dissociation constants of antigen—antibody interactions using data from enzyme-linked immunosorbent assays ELISA [J.
Buy Premium - Download all content for free. The applicability and limitations of the procedure are discussed on the basis of both the experimental and theoretical data.
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POV videos February 24th, In addition, determination of the concentration of specific antibody in the serum is not necessary since the equations use only the ratio of serum concentration and apparent dissociation constant of the immunocomplex both of which can be expressed as serum dilutions.
ELISA enzyme-linked immunosorbent assay.
Methods ]. Here, we present a new linearization procedure-based partly on the former one—to evaluate quantitatively the data of displacement ELISAs. This site does not store any files on its server.
While we were out here, we got the genius idea of shooting porn. It renders possible the determination of dissociation constants of complexes formed between immobilised antigens and the displacing molecules without knowing the explicit concentration of the antigen and the dissociation constant and stoichiometry of the immunocomplex.
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